Investigating the response of cuproproteins from oysters (Crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Metallomics Année : 2014

Investigating the response of cuproproteins from oysters (Crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches

Résumé

The pacific oyster (Crassostrea gigas), a good model for studying biomineralization and coastal environmental change, was used to study the expression of cuproproteins after waterborne Cu exposure (3 μg Cu L(-1)) similarly to the Arcachon Bay aquatic environment. Cuproproteins in oyster cytosols were analyzed by gel electrophoresis (non-denaturing PAGE and SDS 2-DE), laser ablation inductively coupled plasma mass spectrometry (LA-ICP MS) and electrospray ionization mass spectrometry (ESI-MS). The high molecular weight cuproproteins (identified as extracellular superoxide dismutase [Cu-Zn] (EC Cu/Zn-SOD) and l-ascorbate oxidase) in digestive glands of oysters were differentially expressed after Cu exposure. About 1.7 and 1.2-fold increase of the Cu level in EC Cu/Zn-SOD and l-ascorbate oxidase were, respectively, determined, although EC Cu/Zn-SOD was 1.6-1.9-fold down-regulated in the digestive gland of oysters after Cu exposure. At last, the role of EC Cu/Zn-SOD in protection against Cu exposure was discussed to propose possible mechanisms to better understand Cu homeostasis and/or detoxification mechanisms.

Dates et versions

hal-04548196 , version 1 (16-04-2024)

Identifiants

Citer

Ming Xu, Hugues Bijoux, Patrice Gonzalez, Sandra Mounicou. Investigating the response of cuproproteins from oysters (Crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches. Metallomics, 2014, 6 (2), pp.338. ⟨10.1039/c3mt00375b⟩. ⟨hal-04548196⟩

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