Abstract The biological profile estimation is the first step towards positive identification. However, it is not always possible to access a complete and well-preserved skeleton due to postmortem damage and taphonomic changes. As such, there is a need to develop new alternative to analyze different bones of the human skeleton. The present study aims to analyze the patellar osteometry, with attention to its degree of sexual dimorphism, to establish a simple method for estimating sex in Portuguese adults. Six measurements were taken from 222 patella pairs, 117 females and 105 males from the XXI Century Identified Skeleton Collection of the University of Coimbra. Subsequently, this method was validated in a different sample of 50 individuals equally representing both sexes. Maximum height stands out with a 77.0% of correct sex estimation, reaching 98.0% when applied to the new sample. The linear discriminant function analysis containing all the six variables showed the best results, with 80.2% of correct classification after cross-validation and 96.0% when applied to the independent sample.