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Article Dans Une Revue Veterinary Research Année : 2024

A porcine kidney-derived clonal cell line with clear genetic annotation is highly susceptible to African swine fever virus

Hua Cao
  • Fonction : Auteur
Mengjia Zhang
  • Fonction : Auteur
Zheyu Liao
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Dongfan Li
  • Fonction : Auteur
Xinglin He
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Hailong Ma
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Pengfei Li
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Xuexiang Yu
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Guiqing Peng
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Shengsong Xie
  • Fonction : Auteur
Qigai He
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Wentao Li
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Résumé

AbstractAfrican Swine Fever virus (ASFV), the causative agent of African swine fever, is a highly lethal hemorrhagic virus affecting domestic pigs and wild boars. The primary target cells for ASFV infection are porcine alveolar macrophages (PAMs), which are difficult to obtain and maintain in vitro, and less subjective to genetic editing. To overcome these issues and facilitate ASFV research, we obtained a subclonal cell line PK1-C5 by subcloning LLC-PK1 cells that support stable ASFV proliferation. This consequential cell line exhibited high ASFV infection levels and similar viral growth characteristics to PAMs, while also allowing high-efficiency genomic editing through transfection or lentivirus transduction of Cas9. Taken together, our study provided a valuable tool for research aspects including ASFV-host interactions, pathogenicity, and vaccine development.
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Origine : Publication financée par une institution

Dates et versions

hal-04536144 , version 1 (08-04-2024)

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Hua Cao, Mengjia Zhang, Zheyu Liao, Dongfan Li, Xinglin He, et al.. A porcine kidney-derived clonal cell line with clear genetic annotation is highly susceptible to African swine fever virus. Veterinary Research, 2024, 55 (1), pp.42. ⟨10.1186/s13567-024-01300-2⟩. ⟨hal-04536144⟩

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