Bovine-associated non-aureus staphylococci suppress Staphylococcus aureus biofilm dispersal in vitro yet not through agr regulation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Veterinary Research Année : 2021

Bovine-associated non-aureus staphylococci suppress Staphylococcus aureus biofilm dispersal in vitro yet not through agr regulation

Fernando N. de Souza
  • Fonction : Auteur
Kristien Mertens
  • Fonction : Auteur
Sofie Piepers
  • Fonction : Auteur
Freddy Haesebrouck
  • Fonction : Auteur
Sarne De Vliegher
  • Fonction : Auteur

Résumé

AbstractBiofilm formation is a significant virulence factor in Staphylococcus (S.) aureus strains causing subclinical mastitis in dairy cows. A role of environmental signals and communication systems in biofilm development, such as the agr system in S. aureus, is suggested. In the context of multispecies biofilm communities, the presence of non-aureus staphylococci (NAS) might influence S. aureus colonization of the bovine mammary gland, yet, such interspecies interactions have been poorly studied. We determined whether 34 S. chromogenes, 11 S. epidermidis, and 14 S. simulans isolates originating from bovine milk samples and teat apices (TA) were able to affect biofilm formation and dispersion of S. aureus, and if so, how isolate traits such as the capacity to regulate the S. aureus agr quorum sensing system are determinants in this process. The capacity of an agr-positive S. aureus strain to form biofilm was increased more in the presence of S. chromogenes than in the presence of S. simulans and S. epidermidis isolates and in the presence of NAS isolates that do not harbor biofilm related genes. On the other hand, biofilm dispersion of this particular S. aureus strain was suppressed by NAS as a group, an effect that was more pronounced by isolates from TA. Furthermore, the observed effects on biofilm formation and dispersion of the agr-positive S. aureus strain as well as of an agr-negative S. aureus strain did not depend on the capacity of NAS to repress the agr system.
Fichier principal
Vignette du fichier
13567_2021_Article_985.pdf (965.43 Ko) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-04531290 , version 1 (03-04-2024)

Identifiants

Citer

Bruno Toledo-Silva, Fernando N. de Souza, Kristien Mertens, Sofie Piepers, Freddy Haesebrouck, et al.. Bovine-associated non-aureus staphylococci suppress Staphylococcus aureus biofilm dispersal in vitro yet not through agr regulation. Veterinary Research, 2021, 52 (1), pp.114. ⟨10.1186/s13567-021-00985-z⟩. ⟨hal-04531290⟩

Collections

ARINRAE-VR ARINRAE
1 Consultations
3 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More