Slow-wave oscillation in the claustrum in vitro emerges from inhibitory and excitatory cells interactions - Archive ouverte HAL
Poster De Conférence Année : 2020

Slow-wave oscillation in the claustrum in vitro emerges from inhibitory and excitatory cells interactions

Thierry Bal
  • Fonction : Auteur
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Gilles Ouanounou

Résumé

The claustrum and the endopiriform nucleus form a complex with a sheet-like aspect that lay below the insular cortex. It contains various types of interneurons and far-projecting excitatory claustrocortical neurons that makes it the most connected structure in the brain, but its functional role remains largely unknown. Among other propositions, the claustrum is viewed as an integrator for the unified sense of cognition (Crick & Koch, 2005), a hub for attention (Atlan et al., 2018), and may modulate the synchronization of neocortical slow-wave activity (Narikiyo et al., bioarXiv 2018). In a mouse brain slice that contains parts of the claustrum and cortex, we found that claustral principal cells undergo a synchronized and regular 0,3-1 Hz network oscillation under application of the cholinergic agonist carbachol. Light-induced release of acetylcholine from channelrhodopsin-containing cholinergic afferents boosts the oscillation. In order to unravel the mechanisms of the oscillation we performed voltage-clamp protocols in neurons from wild-type and transgenic mice. In principal claustral neurons identified by their somatic shape and electrophysiological characteristics, we found a clear rhythmicity of inhibitions, while excitatory post-synaptic currents distribution remained mostly non-rhythmic. In current-clamp, rhythmic burst firing of action potentials in principal cells was generated by excitatory inputs and terminated by inhibitions. These experiments suggest a pacemaker role for inhibitory neurons under the influence of cholinergic modulation of the network. The pacemaker interneurons within the claustrum remain to be identified, and possible synaptic loops between the claustrum and cortex such as the adjacent insular cortex remains to be ruled out.
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Dates et versions

hal-04530840 , version 1 (03-04-2024)

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  • HAL Id : hal-04530840 , version 1

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Thierry Bal, Gilles Ouanounou. Slow-wave oscillation in the claustrum in vitro emerges from inhibitory and excitatory cells interactions. FENS 2020 virtual Forum Glasgow, Jul 2020, Glasgow, United Kingdom. ⟨hal-04530840⟩
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