Cholinergic induced slow wave oscillation in the claustrum in vitro - Archive ouverte HAL
Poster De Conférence Année : 2019

Cholinergic induced slow wave oscillation in the claustrum in vitro

Résumé

The claustrum (and the endopiriform nucleus) form a complex containing a small proportion of interneurons and a majority of far-projecting excitatory claustrocortical neurons that lay deep below the insular cortex. It is the most reciprocally connected structure in the brain and receives cholinergic and a variety of neuromodulatory inputs from subcortical structures (Goll et al., 2015). Its functional role remains largely unknown. The claustrum has been proposed to be a central integrator for the unified sense of cognition (Crick & Koch, 2005), a hub for attention (Atlan et al., 2018), for multi-sensory binding, and may modulate the synchronization of neocortical slow-wave activity (Narikiyo et al., bioarXiv 2018) or enhance synchronized oscillations between cortical areas (see Vidyasagar & Levichkina, 2019). Little is known on the claustrum circuit properties or on its cell-types: the membrane and synaptic properties, the sensitivity to neuromodulation, and spontaneous states of activities. We use techniques that preserve recurrent slow oscillations (resembling the in vivo up & down states) in cortical circuits in mouse brain slices. In a slice that contains parts of the claustrum and neocortex, we found that the claustrum generates two different types of activities. i) A spontaneous and moderate spiking discharge in principal neurons and interneurons generated by background synaptic activity appears randomly, can last hundreds of milliseconds to seconds, and returns to quiescent periods. ii) Application of the cholinergic agonist carbachol induces in about half of the experiments a robust 0,3-1 Hz rhythmic recurrent network oscillation characterized by the occurrence of compound excitatory and inhibitory postsynaptic potentials and spike discharge. We are exploring the mechanisms of this claustrum slow-wave activity: Does it result solely from the interactions of neurons within the claustrum, although there are few direct connections between its principal cells (Kim et al., 2016), and/or is it due to synaptic loops between the claustrum and neocortex, such as the adjacent insular cortex, or the entorhinal cortex that spontaneously generates Up & down states in the same slice?
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hal-04530830 , version 1 (25-04-2024)

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  • HAL Id : hal-04530830 , version 1

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Thierry Bal, Cyril Monier, Gilles R Ouanounou. Cholinergic induced slow wave oscillation in the claustrum in vitro. Annual Meeting of the Society for Neuroscience, Neuroscience 2019, Oct 2019, Chicago (IL), United States. ⟨hal-04530830⟩
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