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Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2022

Dynamic gene expression and growth underlie cell-to-cell heterogeneity in Escherichia coli stress response

Nadia Sampaio
  • Fonction : Auteur
Caroline Blassick
Jean-Baptiste Lugagne
  • Fonction : Auteur
Mary Dunlop

Résumé

Significance Individual bacteria that share identical genomes and growth environments can display substantial cell-to-cell differences in expression of stress-response genes and single-cell growth rates. This phenotypic heterogeneity can impact the survival of single cells facing sudden stress. However, the windows of time that cells spend in vulnerable or tolerant states are often unknown. We quantify the temporal expression of a suite of stress-response reporters, while simultaneously monitoring growth. We observe pulsatile expression across genes with a range of stress-response functions, finding that single-cell growth rates are often anticorrelated with reporter levels. These dynamic phenotypic differences have a concrete link to function, in which individual cells undergoing a pulse of elevated expression and slow growth are predisposed to survive antibiotic exposure.

Dates et versions

hal-04528200 , version 1 (31-03-2024)

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Citer

Nadia Sampaio, Caroline Blassick, Virgile Andreani, Jean-Baptiste Lugagne, Mary Dunlop. Dynamic gene expression and growth underlie cell-to-cell heterogeneity in Escherichia coli stress response. Proceedings of the National Academy of Sciences of the United States of America, 2022, 119 (14), ⟨10.1073/pnas.2115032119⟩. ⟨hal-04528200⟩
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