Chemical characterization of polysaccharides from Gracilaria gracilis from Bizerte (Tunisia) - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue International Journal of Biological Macromolecules Année : 2024

Chemical characterization of polysaccharides from Gracilaria gracilis from Bizerte (Tunisia)

Résumé

Polysaccharides were extracted from Gracilaria gracilis collected from Manzel Jemil Lake in Bizerte Tunisia, with two different solvents (water and NaOH 0.3 M). Different assays were performed on samples (total sugars, neutral sugars, uronic acids, anhydrogalactose, proteins, sulphates, pyruvates), followed by high performance anion-exchange chromatography (HPAEC) to observe the monosaccharide composition, high pressure size exclusion chromatography with multi-angle laser light scattering (HPSEC-MALS) to obtain the molecular mass, Fourier transform infrared spectroscopy (FTIR), and 1D and 2D nuclear magnetic resonance (NMR) to access to structural data. Results have shown that the polysaccharide extracted from Gracilaria gracilis collected from Manzel Jemil Lake in Bizerte Tunisia, is of agar type but with high molecular mass and some original structural features. Hence, the sample was found to contain 9 % of pyruvate groups and is partly sulphated at the C4 of β-d-galactose and methylated on C2 of anhydro-α-l-galactose. The polymer from G. gracilis from Bizerte thus presents a never described structure that could be interesting for further rheological or biological activities applications.
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Dates et versions

hal-04524825 , version 1 (28-03-2024)

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Citer

K. Yahyaoui, M. Traikia, C. Rihouey, L. Picton, C. Gardarin, et al.. Chemical characterization of polysaccharides from Gracilaria gracilis from Bizerte (Tunisia). International Journal of Biological Macromolecules, 2024, pp.131127. ⟨10.1016/j.ijbiomac.2024.131127⟩. ⟨hal-04524825⟩
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