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Article Dans Une Revue Methods in Molecular Biology Année : 2024

Isolation of the Inner and Outer Membranes of the Chloroplast Envelope from Angiosperms

Maryse Block
  • Fonction : Auteur
Eric Maréchal

Résumé

The outer and the inner membranes of the chloroplast envelope, also called OEM and IEM, have distinct lipid and protein compositions. They host molecular systems involved in the biogenesis of the organelle, its cellular function, and its communication with other compartments. Here we describe a method for the isolation of these two membranes starting from intact chloroplast preparations, with two alternative procedures based on the starting material. One was developed from spinach leaves, the other from pea leaves. The two procedures differ in the method used to isolate and rupture chloroplasts and separate each membrane.
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mardi 24 septembre 2024
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Dates et versions

hal-04520798 , version 1 (25-03-2024)

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Maryse Block, Eric Maréchal. Isolation of the Inner and Outer Membranes of the Chloroplast Envelope from Angiosperms. Methods in Molecular Biology, 2024, Plastids. Methods and Protocols, 2776, pp.151-159. ⟨10.1007/978-1-0716-3726-5_8⟩. ⟨hal-04520798⟩
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