Quantitative MRI of Gd‐DOTA Accumulation in the Mouse Brain After Intraperitoneal Administration: Validation by Mass Spectrometry - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Magnetic Resonance Imaging Année : 2023

Quantitative MRI of Gd‐DOTA Accumulation in the Mouse Brain After Intraperitoneal Administration: Validation by Mass Spectrometry

Résumé

Background In mice, intraperitoneal (ip) contrast agent (CA) administration is convenient for mapping microvascular parameters over a long‐time window. However, continuous quantitative MRI of CA accumulation in brain over hours is still missing. Purpose To validate a quantitative time‐resolved MRI technique for mapping the CA kinetics in brain upon ip administration. Study Type Prospective, animal model. Specimen 25 C57Bl/6JRj mice underwent MRI. Field Strength/Sequence 7‐T, gradient echo sequence. Assessment Gd‐DOTA concentration was monitored by MRI (25 s/repetition) over 135 minutes with (N = 15) and without (N = 10) ip mannitol challenge (5 g/kg). After the final repetition, the brains were sampled to quantify gadolinium by mass spectrometry (MS). Upon manual brain segmentation, the average gadolinium concentration was compared with the MS quantification in transcardially perfused (N = 20) and unperfused (N = 5) mice. Precontrast T 1 ‐maps were acquired in 8 of 25 mice. Statistical Tests One‐tailed Spearman and Pearson correlation between gadolinium quantification by MRI and by MS, D'Agostino‐Pearson test for normal distribution, Bland–Altman analysis to evaluate the agreement between MRI and MS. Significance was set at P ‐value <0.05. Results MRI showed that ip administered CA reached the blood compartment (>5 mM) within 10 minutes and accumulated continuously for 2 hours in cerebrospinal fluid (>1 mM) and in brain tissue. The MRI‐derived concentration maps showed interindividual differences in CA accumulation (from 0.47 to 0.81 mM at 2 hours) with a consistent distribution resembling the pathways of the glymphatic system. The average in‐vivo brain concentration 2 hours post‐CA administration correlated significantly ( r = 0.8206) with the brain gadolinium quantification by MS for N = 21 paired observations available. Data Conclusion The presented experimental and imaging protocol may be convenient for monitoring the spatiotemporal pattern of CA uptake and clearance in the mouse brain over 2 hours. The quantification of the CA from the MRI signal in brain is corroborated by MS. Evidence Level N/A Technical Efficacy Stage 1
Fichier principal
Vignette du fichier
Magnetic Resonance Imaging - 2023 - Quantitative MRI of Gd‐DOTA Accumulation in the Mouse Brain After.pdf (2.27 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Licence : CC BY - Paternité

Dates et versions

hal-04520141 , version 1 (25-03-2024)

Licence

Paternité

Identifiants

Citer

Anthony Tessier, Anthony Ruze, Isabelle Varlet, Estelle M.H. Laïb, Emilien Royer, et al.. Quantitative MRI of Gd‐DOTA Accumulation in the Mouse Brain After Intraperitoneal Administration: Validation by Mass Spectrometry. Journal of Magnetic Resonance Imaging, 2023, ⟨10.1002/jmri.29034⟩. ⟨hal-04520141⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More