The role of PB1-F2 in adaptation of high pathogenicity avian influenza virus H7N7 in chickens - Archive ouverte HAL
Article Dans Une Revue Veterinary Research Année : 2024

The role of PB1-F2 in adaptation of high pathogenicity avian influenza virus H7N7 in chickens

Luise Hohensee
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David Scheibner
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Alexander Schäfer
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Holly Shelton
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Thomas C. Mettenleiter
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Angele Breithaupt
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Anca Dorhoi
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Elsayed M. Abdelwhab
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Ulrike Blohm
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Résumé

AbstractAvian influenza viruses (AIV) of the H7N7 subtype are enzootic in the wild bird reservoir in Europe, cause infections in poultry, and have sporadically infected humans. The non-structural protein PB1-F2 is encoded in a second open frame in the polymerase segment PB1 and its sequence varies with the host of origin. While mammalian isolates predominantly carry truncated forms, avian isolates typically express full-length PB1-F2. PB1-F2 is a virulence factor of influenza viruses in mammals. It modulates the host immune response, causing immunopathology and increases pro-inflammatory responses. The role of full-length PB1-F2 in IAV pathogenesis as well as its impact on virus adaptation and virulence in poultry remains enigmatic. Here, we characterised recombinant high pathogenicity AIV (HPAIV) H7N7 expressing or lacking PB1-F2 in vitro and in vivo in chickens. In vitro, full-length PB1-F2 modulated viability of infected chicken fibroblasts by limiting apoptosis. In chickens, PB1-F2 promoted gastrointestinal tropism, as demonstrated by enhanced viral replication in the gut and increased cloacal shedding. PB1-F2’s effects on cellular immunity however were marginal. Overall, chickens infected with full-length PB1-F2 virus survived for shorter periods, indicating that PB1-F2 is also a virulence factor in bird-adapted viruses.
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Dates et versions

hal-04519614 , version 1 (25-03-2024)

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Luise Hohensee, David Scheibner, Alexander Schäfer, Holly Shelton, Thomas C. Mettenleiter, et al.. The role of PB1-F2 in adaptation of high pathogenicity avian influenza virus H7N7 in chickens. Veterinary Research, 2024, 55 (1), pp.5. ⟨10.1186/s13567-023-01257-8⟩. ⟨hal-04519614⟩

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