Immunodetection of NUT Protein: Implementation, Indications, and Results in a Tertiary Reference Center - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Applied Immunohistochemistry and Molecular Morphology Année : 2024

Immunodetection of NUT Protein: Implementation, Indications, and Results in a Tertiary Reference Center

Résumé

The immunodetection of NUT protein is a reliable tool to identify NUT carcinoma, a rare and still underdiagnosed tumor entity. The technique was implemented in 2017 in our department, a tertiary reference center with a large recruitment in all tumor types, including head and neck and thoracic tumors. We evaluated its use over a 6-year period (2017–2022) to (a) describe the indications for the technique, (b) determine the number of NUT carcinomas detected and confirmed by Fluorescence in situ hybridization, and (c) describe briefly the characteristics of these tumors. Over the study period, 382 NUT immunodetections were performed; the annual number of requests varied from 45 to 83. All 21 pathologists of the department made at least one request (range: 1 to 94; annual mean: 18.2). 54.7% of immunodetections were performed for internal cases, 37% for cases submitted for consultation, and 8.3% for cases submitted for confirmation of a suspected diagnosis. The main indications were poorly differentiated tumors of the head and neck region (39%) and the thorax (19.6%), and difficult-to-classify soft tissue tumors (11.8%). Twelve cases of NUT carcinoma were detected by immunohistochemistry and confirmed by Fluorescence in situ hybridization. Seven were from the head and neck region (4.7% of the tumors tested), 4 from lung or mediastinum (5.3%), 1 from an unknown primary at the time of diagnosis. In conclusion, the implementation of NUT immunodetection in the daily workflow of a pathology department improves the detection of NUT carcinoma. This becomes essential with the emergence of potential targeted therapies.
Fichier non déposé

Dates et versions

hal-04517594 , version 1 (22-03-2024)

Identifiants

Citer

Hussain Noorwali, Odile Casiraghi, Marion Classe, Julien Adam, Carine Ngo, et al.. Immunodetection of NUT Protein: Implementation, Indications, and Results in a Tertiary Reference Center. Applied Immunohistochemistry and Molecular Morphology, 2024, 32 (2), pp.64-70. ⟨10.1097/PAI.0000000000001172⟩. ⟨hal-04517594⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More