In vitro and in vivo models define a molecular signature reference for human embryonic notochordal cells - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue iScience Année : 2024

In vitro and in vivo models define a molecular signature reference for human embryonic notochordal cells

Résumé

Understanding the emergence of human notochordal cells (NC) is essential for the development of regenerative approaches. We present a comprehensive investigation into the specification and generation of bona fide NC using a straightforward pluripotent stem cell (PSC)-based system benchmarked with human fetal notochord. By integrating in vitro and in vivo transcriptomic data at single-cell resolution, we establish an extended molecular signature and overcome the limitations associated with studying human notochordal lineage at early developmental stages. We show that TGF-β inhibition enhances the yield and homogeneity of notochordal lineage commitment in vitro. Furthermore, this study characterizes regulators of cell-fate decision and matrisome enriched in the notochordal niche. Importantly, we identify specific cell-surface markers opening avenues for differentiation refinement, NC purification, and functional studies. Altogether, this study provides a human notochord transcriptomic reference that will serve as a resource for notochord identification in human systems, diseased-tissues modeling, and facilitating future biomedical research.

Dates et versions

hal-04517538 , version 1 (22-03-2024)

Identifiants

Citer

Julie Warin, Nicolas Vedrenne, Vivian Tam, Mengxia Zhu, Danqing Yin, et al.. In vitro and in vivo models define a molecular signature reference for human embryonic notochordal cells. iScience, 2024, 27 (2), pp.109018. ⟨10.1016/j.isci.2024.109018⟩. ⟨hal-04517538⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More