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Article Dans Une Revue Biomolecular NMR Assignments Année : 2023

Backbone NMR resonance assignment of the apo human Tsg101-UEV domain

Résumé

The Endosomal Sorting Complex Required for Transport (ESCRT) pathway, through inverse topology membrane remodeling, is involved in many biological functions, such as ubiquitinated membrane receptor trafficking and degradation, multivesicular bodies (MVB) formation and cytokinesis. Dysfunctions in ESCRT pathway have been associated to several human pathologies, such as cancers and neurodegenerative diseases. The ESCRT machinery is also hijacked by many enveloped viruses to bud away from the plasma membrane of infected cells. Human tumor susceptibility gene 101 (Tsg101) protein is an important ESCRT-I complex component. The structure of the N-terminal ubiquitin E2 variant (UEV) domain of Tsg101 (Tsg101-UEV) comprises an ubiquitin binding pocket next to a late domain [P(S/T)AP] binding groove. These two binding sites have been shown to be involved both in the physiological roles of ESCRT-I and in the release of the viral particles, and thus are attractive targets for antivirals. The structure of the Tsg101-UEV domain has been characterized, using X-ray crystallography or NMR spectroscopy, either in its apo-state or bound to ubiquitin or late domains. In this study, we report the backbone NMR resonance assignments, including the proline signals, of the apo human Tsg101-UEV domain, that so far was not publicly available. These data, that are in good agreement with the crystallographic structure of Tsg101-UEV domain, can therefore be used for further NMR studies, including protein-protein interaction studies and drug discovery.
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Dates et versions

hal-04515138 , version 1 (21-03-2024)

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Danai Moschidi, François-Xavier Cantrelle, Emmanuelle Boll, Xavier Hanoulle. Backbone NMR resonance assignment of the apo human Tsg101-UEV domain. Biomolecular NMR Assignments, 2023, 17 (1), pp.49-54. ⟨10.1007/s12104-023-10119-5⟩. ⟨hal-04515138⟩
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