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Article Dans Une Revue (Article De Synthèse) International Journal of Molecular Sciences Année : 2024

State-of-the-Art Differentiation Protocols for Patient-Derived Cardiac Pacemaker Cells

Résumé

Human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes raise the possibility of generating pluripotent stem cells from a wide range of human diseases. In the cardiology field, hiPSCs have been used to address the mechanistic bases of primary arrhythmias and in investigations of drug safety. These studies have been focused primarily on atrial and ventricular pathologies. Consequently, many hiPSC-based cardiac differentiation protocols have been developed to differentiate between atrial- or ventricular-like cardiomyocytes. Few protocols have successfully proposed ways to obtain hiPSC-derived cardiac pacemaker cells, despite the very limited availability of human tissues from the sinoatrial node. Providing an in vitro source of pacemaker-like cells would be of paramount importance in terms of furthering our understanding of the mechanisms underlying sinoatrial node pathophysiology and testing innovative clinical strategies against sinoatrial node dysfunction (i.e., biological pacemakers and genetic- and pharmacological- based therapy). Here, we summarize and detail the currently available protocols used to obtain patient-derived pacemaker-like cells.
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Dates et versions

hal-04510562 , version 1 (19-03-2024)

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Eleonora Torre, Matteo Mangoni, Alain Lacampagne, Albano Meli, Pietro Mesirca. State-of-the-Art Differentiation Protocols for Patient-Derived Cardiac Pacemaker Cells. International Journal of Molecular Sciences, 2024, 25 (6), pp.3387. ⟨10.3390/ijms25063387⟩. ⟨hal-04510562⟩
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