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Communication Dans Un Congrès Année : 2018

Exchange of metabolites and metabolic signals between plastids and cytosol and novel pathways of protein sorting towards plastids using the QORH protein as model

Résumé

Chloroplasts are a major component of plant cells. Their origin traces back to a cyanobacterial ancestor that was engulfed by an ancient eukaryotic cell and eventually integrated as an organelle during evolution. As a result, more than 95% of the ancestral cyanobacterial genes were transferred to the host cell nucleus. Proteins encoded by these relocated genes need to return to internal chloroplast compartments. This import is mainly achieved by the general TOC/TIC machinery located at the chloroplast surface. Until recently, all proteins destined to chloroplast were believed to possess an N-terminal and cleavable chloroplast targeting peptide, and to engage the TOC/TIC machinery. However, recent studies have revealed the existence of non-canonical preproteins, lacking cleavable transit peptides. Furthermore, few such proteins were demonstrated to use alternative targeting pathways, independent of the TOC/TIC machinery. The aim of our project aims at deciphering the molecular nature of these alternative targeting machineries. For that, we initiated a targeted study combining affinity purification and mass spectrometry aiming to identify alternative receptors at the chloroplast surface. Alternatively, we revisited the envelope proteome composition and initiated a gene candidate approach. We are currently studying the role of putative import receptors using in planta techniques.
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Dates et versions

hal-04509380 , version 1 (18-03-2024)

Identifiants

  • HAL Id : hal-04509380 , version 1

Citer

Imen Bouchnak, Lucas Moyet, Daniel Salvi, Sabine Brugière, Marcel Kuntz, et al.. Exchange of metabolites and metabolic signals between plastids and cytosol and novel pathways of protein sorting towards plastids using the QORH protein as model. Jacques-Monod-Conference on "Retrograde signalling from endosymbiotic organelles", Thomas Pfannschmidt, Matthew Terry, Oct 2018, Roscoff, France. ⟨hal-04509380⟩
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