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Article Dans Une Revue Veterinary Research Année : 2024

Construction of recombinant fluorescent LSDV for high-throughput screening of antiviral drugs

Jingyu Wang
Jinzhao Ji
  • Fonction : Auteur
Yongcheng Zhong
  • Fonction : Auteur
Wenxin Meng
  • Fonction : Auteur
Shaobin Wan
  • Fonction : Auteur
Xiaoqing Ding
  • Fonction : Auteur
Zihan Chen
  • Fonction : Auteur
Weiyong Wu
  • Fonction : Auteur
Kun Jia
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  • PersonId : 1364514

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Shoujun Li
  • Fonction : Auteur correspondant
  • PersonId : 1364515

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Résumé

AbstractLumpy skin disease virus (LSDV) infection is a major socio-economic issue that seriously threatens the global cattle-farming industry. Here, a recombinant virus LSDV-ΔTK/EGFP, expressing enhanced green fluorescent protein (EGFP), was constructed with a homologous recombination system and applied to the high-throughput screening of antiviral drugs. LSDV-ΔTK/EGFP replicates in various kidney cell lines, consistent with wild-type LSDV. The cytopathic effect, viral particle morphology, and growth performance of LSDV-ΔTK/EGFP are consistent with those of wild-type LSDV. High-throughput screening allowed to identify several molecules that inhibit LSDV-ΔTK/EGFP replication. The strong inhibitory effect of theaflavin on LSDV was identified when 100 antiviral drugs were screened in vitro. An infection time analysis showed that theaflavin plays a role in the entry of LSDV into cells and in subsequent viral replication stages. The development of this recombinant virus will contribute to the development of LSDV-directed antiviral drugs and the study of viral replication and mechanisms of action.
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Dates et versions

hal-04508412 , version 1 (18-03-2024)

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Jingyu Wang, Jinzhao Ji, Yongcheng Zhong, Wenxin Meng, Shaobin Wan, et al.. Construction of recombinant fluorescent LSDV for high-throughput screening of antiviral drugs. Veterinary Research, 2024, 55 (1), pp.33. ⟨10.1186/s13567-024-01281-2⟩. ⟨hal-04508412⟩

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