Article Dans Une Revue NeuroImage Année : 2022

Long-term deep intracerebral microelectrode recordings in patients with drug-resistant epilepsy: Proposed guidelines based on 10-year experience

Résumé

Purpose: Human neuronal activity, recorded in vivo from microelectrodes, may offer valuable insights into phys- iological mechanisms underlying human cognition and pathophysiological mechanisms of brain diseases, in par- ticular epilepsy. Continuous and long-term recordings are necessary to monitor non predictable pathological and physiological activities like seizures or sleep. Because of their high impedance, microelectrodes are more sensitive to noise than macroelectrodes. Low noise levels are crucial to detect action potentials from background noise, and to further isolate single neuron activities. Therefore, long-term recordings of multi-unit activity remains a challenge. We shared here our experience with microelectrode recordings and our efforts to reduce noise levels in order to improve signal quality. We also provided detailed technical guidelines for the connection, recording, imaging and signal analysis of microelectrode recordings. Results: During the last 10 years, we implanted 122 bundles of Behnke-Fried hybrid macro-microelectrodes, in 56 patients with pharmacoresistant focal epilepsy. Microbundles were implanted in the temporal lobe (74%), as well as frontal (15%), parietal (6%) and occipital (5%) lobes. Low noise levels depended on our technical setup. The noise reduction was mainly obtained after electrical insulation of the patient’s recording room and the use of a reinforced microelectrode model, reaching median root mean square values of 5.8 μV. Seventy percent of the bundles could record multi-units activities (MUA), on around 3 out of 8 wires per bundle and for an average of 12 days. Seizures were recorded by microelectrodes in 91% of patients, when recorded continuously, and MUA were recorded during seizures for 75 % of the patients after the insulation of the room. Technical guidelines are proposed for (i) electrode tails manipulation and protection during surgical bandage and connection to both clinical and research amplifiers, (ii) electrical insulation of the patient’s recording room and shielding, (iii) data acquisition and storage, and (iv) single-units activities analysis

Fichier principal
Vignette du fichier
1-s2.0-S1053811922002440-main.pdf (3.08 Mo) Télécharger le fichier
Origine Publication financée par une institution
Licence

Dates et versions

hal-04505896 , version 1 (15-03-2024)

Licence

Identifiants

Citer

Katia Lehongre, Virginie Lambrecq, Stephen Whitmarsh, Valerio Frazzini, Louis Cousyn, et al.. Long-term deep intracerebral microelectrode recordings in patients with drug-resistant epilepsy: Proposed guidelines based on 10-year experience. NeuroImage, 2022, 254, pp.119116. ⟨10.1016/j.neuroimage.2022.119116⟩. ⟨hal-04505896⟩
105 Consultations
212 Téléchargements

Altmetric

Partager

  • More