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Communication Dans Un Congrès Année : 2023

Chloroplast biogenesis: towards the role of localized translation in Arabidopsis

Sabine Brugière

Résumé

Chloroplasts are a major component of plant cells. Until recently, all nuclear-encoded proteins destined to chloroplast were believed to possess an N-terminal and cleavable chloroplast targeting peptide, and to engage the TOC/TIC machinery. However, recent studies have revealed that alternative routes also exists and identified a series of nuclear-encoded proteins imported via such pathways. Recent proteomic studies, conducted by our team (Bouchnak et al., Mol Cell Proteomics 2019), identified a list of cyto-ribosomal subunits associated to chloroplasts, thus suggesting that localized translation might occur at the chloroplast surface. We were recently able to isolate plastid-associated cyto-ribosomes and to decipher their composition when compared to purified whole cell cyto-ribosomes. Interestingly, these plastid-associated cyto-ribosomes contain a few non cyto-ribosomal proteins which might participate to the control of localized translation at the chloroplast surface. Finally, with the aim to identify the nature of the nuclear-encoded mRNAs that are translated by these chloroplast-associated cyto-ribosomes, the identification of mRNAs trapped within these plastid-associated cyto-ribosomes was performed.
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Dates et versions

hal-04505232 , version 1 (14-03-2024)

Identifiants

  • HAL Id : hal-04505232 , version 1

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Norbert Rolland, Laura Dimnet, Sara Pullara, Lucas Moyet, Chloé Genevey, et al.. Chloroplast biogenesis: towards the role of localized translation in Arabidopsis. 59th Congress of the Argentine Society for Research in Biochemistry and Molecular Biology (SAIB), SAIB, Nov 2023, Rosario, Argentina. ⟨hal-04505232⟩
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