Bio-Guided Fractionation and Molecular Networking Reveal Fatty Acids to Be Principal Anti-Parasitic Compounds in Nordic Seaweeds - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Frontiers in Pharmacology Année : 2021

Bio-Guided Fractionation and Molecular Networking Reveal Fatty Acids to Be Principal Anti-Parasitic Compounds in Nordic Seaweeds

Louis Bornancin
  • Fonction : Auteur
Yi Lu
  • Fonction : Auteur
María Martínez-Valladares
  • Fonction : Auteur
Miguel Peña-Espinoza
  • Fonction : Auteur
Helena Mejer
  • Fonction : Auteur
Andrew Williams
  • Fonction : Auteur
Stig Milan Thamsborg
  • Fonction : Auteur

Résumé

Widespread use of antimicrobial drugs has led to high levels of drug-resistance in pathogen populations and a need for novel sources of anti-bacterial and anti-parasitic compounds. Macroalgae (seaweed) are potentially a rich source of bioactive compounds, and several species have traditionally been used as vermifuges. Here, we investigated the anti-parasitic properties of four common cold-water Nordic seaweeds; Palmaria palmata (Rhodophyta), Laminaria digitata , Saccharina latissima and Ascophyllum nodosum (Ochrophyta, Phaeophyceae). Screening of organic extracts against helminths of swine ( Ascaris suum ) and sheep ( Teladorsagia circumcincta ) revealed that S. latissima and L. digitata had particularly high biological activity. A combination of molecular networking and bio-guided fractionation led to the isolation of six compounds from extracts of these two species identified in both fermented and non-fermented samples. The six isolated compounds were tentatively identified by using MS-FINDER as five fatty acids and one monoglyceride: Stearidonic acid ( 1 ), Eicosapentaenoic acid ( 2 ), Alpha-Linolenic acid ( 3 ), Docosahexaenoic acid ( 4 ), Arachidonic acid ( 5 ), and Monoacylglycerol (MG 20:5) ( 6 ). Individual compounds showed only modest activity against A. suum , but a clear synergistic effect was apparent when selected compounds were tested in combination. Collectively, our data reveal that fatty acids may have a previously unappreciated role as natural anti-parasitic compounds, which suggests that seaweed products may represent a viable option for control of intestinal helminth infections.

Dates et versions

hal-04503384 , version 1 (13-03-2024)

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Citer

Charlotte Smith Bonde, Louis Bornancin, Yi Lu, Henrik Toft Simonsen, María Martínez-Valladares, et al.. Bio-Guided Fractionation and Molecular Networking Reveal Fatty Acids to Be Principal Anti-Parasitic Compounds in Nordic Seaweeds. Frontiers in Pharmacology, 2021, 12, ⟨10.3389/fphar.2021.674520⟩. ⟨hal-04503384⟩
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