The ClC-3 chloride channel and osmoregulation in the European Sea Bass, Dicentrarchus labrax - Archive ouverte HAL
Article Dans Une Revue Journal of Comparative Physiology B Année : 2013

The ClC-3 chloride channel and osmoregulation in the European Sea Bass, Dicentrarchus labrax

Maryline Bossus
  • Fonction : Auteur
Guy Charmantier
  • Fonction : Auteur
Eva Blondeau-Bidet
  • Fonction : Auteur
Bianca Valletta
  • Fonction : Auteur
Catherine Lorin-Nebel
  • Fonction : Auteur

Résumé

Dicentrarchus labrax migrates between sea (SW), brackish and fresh water (FW) where chloride concentrations and requirements for chloride handling change: in FW, fish absorb chloride and restrict renal losses; in SW, they excrete chloride. In this study, the expression and localization of ClC-3 and Na+/K+-ATPase (NKA) were studied in fish adapted to SW, or exposed to FW from 10 min to 30 days. In gills, NKA-alpha 1 subunit expression transiently increased from 10 min and reached a stabilized intermediate expression level after 24 h in FW. ClC-3 co-localized with NKA in the basolateral membrane of mitochondria-rich cells (MRCs) at all conditions. The intensity of MRC ClC-3 immunostaining was significantly higher (by 50 %) 1 h after the transfer to FW, whereas the branchial ClC-3 protein expression was 30 % higher 7 days after the transfer as compared to SW. This is consistent with the increased number of immunopositive MRCs (immunostained for NKA and ClC-3). However, the ClC-3 mRNA expression was significantly lower in FW gills. In the kidney, after FW transfer, a transient decrease in NKA-alpha 1 subunit expression was followed by significantly higher stable levels from 24 h. The low ClC-3 protein expression detected at both salinities was not observed by immunocytochemistry in the SW kidney; ClC-3 was localized in the basal membrane of the collecting ducts and tubules 7 and 30 days after transfer to FW. Renal ClC-3 mRNA expression, however, seemed higher in SW than in FW. The potential role of this chloride channel ClC-3 in osmoregulatory and osmosensing mechanisms is discussed.

Dates et versions

hal-04499399 , version 1 (11-03-2024)

Identifiants

Citer

Maryline Bossus, Guy Charmantier, Eva Blondeau-Bidet, Bianca Valletta, Viviane Boulo, et al.. The ClC-3 chloride channel and osmoregulation in the European Sea Bass, Dicentrarchus labrax. Journal of Comparative Physiology B, 2013, 183 (5), pp.641-662. ⟨10.1007/s00360-012-0737-9⟩. ⟨hal-04499399⟩
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