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Article Dans Une Revue Sensors and Actuators B: Chemical Année : 2023

Sulfur source-mediated in situ growth of highly conductive nanocomposites for sensitive detection of miRNA-21

Résumé

The accurate detection of miRNA-21, one of the emerging biomarkers for breast cancer, is extremely important for facilitating early diagnosis. In this paper, we present a novel nanocomposite material Co9S8@WS2/DWCNT was prepared through a one-pot hydrothermal method. WS2 (Tungsten disulfide) semiconductor nanosheets were used as negative carriers to provide binding sites. TAA (Thioacetamide) was used as a sulfur source and crosslinking agent to modify ZIF-67 (Co-based zeolite imidazole framework) into Co9S8 with excellent electrical conductivity and to grow it in situ on the WS2 surface. DWCNT (Double-walled carbon nanotubes) was then stacked with WS2 by π-π conjugation. To further enhance the electrical conductivity and biocompatibility of the composite nanomaterials, it was doped by AgNPs (Silver nanoparticles) to obtain Co9S8@WS2/DWCNT-AgNPs nanocomposites with excellent electrochemical properties. The capture probe cDNA was immobilized on the electrode through Ag-S bonding to enable sensitive detection of miRNA-21. With a concentration response range of 10^-18 M - 10^-11 M and a detection limit as low as 1.54 aM, the detection of miRNA-21 in human serum samples has been successfully achieved applying to this biosensing platform.
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Dates et versions

hal-04497322 , version 1 (09-03-2024)

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Sinan Fu, Zhizhi Ning, Qiuyue Li, Yutao He, Chang Xie, et al.. Sulfur source-mediated in situ growth of highly conductive nanocomposites for sensitive detection of miRNA-21. Sensors and Actuators B: Chemical, 2023, 392, pp.134086. ⟨10.1016/j.snb.2023.134086⟩. ⟨hal-04497322⟩
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