Electrochemical signal amplification strategy based on trace metal ion modified WS2 for ultra-sensitive detection of miRNA-21 - Archive ouverte HAL Access content directly
Journal Articles Talanta Year : 2023

Electrochemical signal amplification strategy based on trace metal ion modified WS2 for ultra-sensitive detection of miRNA-21

Abstract

Ochratoxin A (OTA) is considered the most toxic member of the ochratoxin group. Herein, a novel label-free electrochemical sensor based on the horseradish peroxidase (HRP) enzyme is developed for OTA detection. The HRP enzyme was covalently immobilized on the working electrode of a planar boron-doped diamond (BDD) electrochemical microcell previously covered with diazonium film and grafted with single-walled carbon nanotubes (SWCNTs). Each surface modification step was evaluated by cyclic voltammetry and scanning electron microscopy. Square wave voltammetry was used for the detection of OTA. The linear working range of the biosensors ranged between 10−14 and 0.1 M, with a limit of detection (LOD) of 10 fM, an RSD equal to 5%, and a sensitivity of 0.8 µA per decade. In addition, the sensor showed good selectivity in the presence of OTA analogs; it was validated in samples such as corn, feed, and wheat. The metrological performance of the present sensor makes it a good alternative for OTA detection.
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Dates and versions

hal-04497298 , version 1 (09-03-2024)

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Sinan Fu, Chang Xie, Zhiruo Yang, Mingdi Jiang, Jing Cheng, et al.. Electrochemical signal amplification strategy based on trace metal ion modified WS2 for ultra-sensitive detection of miRNA-21. Talanta, 2023, 260 (3), pp.124552. ⟨10.1016/j.talanta.2023.124552⟩. ⟨hal-04497298⟩
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