Activin A Modulates CRIPTO-1/HNF4 α + Cells to Guide Cardiac Differentiation from Human Embryonic Stem Cells - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Stem Cells International Année : 2017

Activin A Modulates CRIPTO-1/HNF4 α + Cells to Guide Cardiac Differentiation from Human Embryonic Stem Cells

Robin Duelen
  • Fonction : Auteur
Abdulsamie Patel
  • Fonction : Auteur
Nathalie de Schaetzen
  • Fonction : Auteur
Liesbeth de Waele
  • Fonction : Auteur
Llewelyn Roderick
  • Fonction : Auteur
Karin Sipido
  • Fonction : Auteur
Catherine Verfaillie
  • Fonction : Auteur
Gunnar Buyse
  • Fonction : Auteur
Lieven Thorrez
Maurilio Sampaolesi

Résumé

The use of human pluripotent stem cells in basic and translational cardiac research requires efficient differentiation protocols towards cardiomyocytes. In vitro differentiation yields heterogeneous populations of ventricular-, atrial-, and nodal-like cells hindering their potential applications in regenerative therapies. We described the effect of the growth factor Activin A during early human embryonic stem cell fate determination in cardiac differentiation. Addition of high levels of Activin A during embryoid body cardiac differentiation augmented the generation of endoderm derivatives, which in turn promoted cardiomyocyte differentiation. Moreover, a dose-dependent increase in the coreceptor expression of the TGF- β superfamily member CRIPTO-1 was observed in response to Activin A. We hypothesized that interactions between cells derived from meso- and endodermal lineages in embryoid bodies contributed to improved cell maturation in early stages of cardiac differentiation, improving the beating frequency and the percentage of contracting embryoid bodies. Activin A did not seem to affect the properties of cardiomyocytes at later stages of differentiation, measuring action potentials, and intracellular Ca 2+ dynamics. These findings are relevant for improving our understanding on human heart development, and the proposed protocol could be further explored to obtain cardiomyocytes with functional phenotypes, similar to those observed in adult cardiac myocytes.

Dates et versions

hal-04491693 , version 1 (06-03-2024)

Identifiants

Citer

Robin Duelen, Guillaume Gilbert, Abdulsamie Patel, Nathalie de Schaetzen, Liesbeth de Waele, et al.. Activin A Modulates CRIPTO-1/HNF4 α + Cells to Guide Cardiac Differentiation from Human Embryonic Stem Cells. Stem Cells International, 2017, 2017, pp.1-17. ⟨10.1155/2017/4651238⟩. ⟨hal-04491693⟩
4 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More