ARE FOODS ENRICHED WITH QUORUM SENSING AUTOINDUCERS CAPABLE OF AFFECTING THE NEONATAL ASSEMBLY OF THE GUT MICROBIOTA IN RATS? - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2023

ARE FOODS ENRICHED WITH QUORUM SENSING AUTOINDUCERS CAPABLE OF AFFECTING THE NEONATAL ASSEMBLY OF THE GUT MICROBIOTA IN RATS?

Résumé

Considering the current craze for new fermented foods and their benefits for health through possible intestinal microbiota modulation [1], we assessed whether the consumption of modelled foods enriched with acyl-homoserine-lactones (AHLs), some quorum sensing molecules regulating cooperative bacterial behaviors, could modulate the early assembly of intestinal microbiota. We, therefore, screened strains of Hafnia alvei (a usual cheese-ripening starter known as an AHLs-producer [2]) for their ability to synthesize AHLs on milk ultrafiltrate. We then generated model foods enriched or not in AHLs by alkalinizing or not filter-sterilized supernatants from strain CIRM-BIA 1832. We then subjected AHLs (mainly 3-oxo-C6-AHL and C6-AHL 85/15) enriched food to an in vitro digestion test [3], which indicated that ca. ¾ of the initial AHLs survived to conditions mimicking digestion. Finally, we typified the impacts of 8 days-supplementation of recently weaned rats (aged 28 to 35 days) with these model foods on their intestinal microbiota [cf 4]. Feeding AHLs supplements had no significant impact on pups growth nor food consumption. Similarly, no significant differences were observed concerning colonic microbiota features (alpha and beta diversities indexes, relative abundances of main families and genera). Analyzes are underway to determine whether these conclusions are valid for the ileal microbiota. [1] Wastyk HC, Fragiadakis GK, Perelman D, et al. Gut-microbiota-targeted diets modulate human immune status. Cell. 2021;184(16):4137-4153.e14. doi: 10.1016/j.cell.2021.06.019. [2] Li X, Zhang G, Zhu Y, et al. Effect of the luxI/R gene on AHL-signaling molecules and QS regulatory mechanism in Hafnia alvei H4. AMB Express. 2019;9(1):197. doi: 10.1186/s13568-019-0917-z. [3] Brodkorb A, Egger L, Alminger M, et al. INFOGEST static in vitro simulation of gastrointestinal food digestion. Nat Protoc. 2019;14(4):991-1014. doi: 10.1038/s41596-018-0119-1. [4] Le Dréan G, Pocheron AL, Billard H, et al. Neonatal Consumption of Oligosaccharides Greatly Increases L-Cell Density without Significant Consequence for Adult Eating Behavior. Nutrients. 2019;11(9):1967. doi: 10.3390/nu11091967.
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Dates et versions

hal-04491014 , version 1 (05-03-2024)

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Paternité - Pas d'utilisation commerciale

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  • HAL Id : hal-04491014 , version 1

Citer

Catherine Michel, Raouf Tareb, Mikael Croyal, Anne Meynier, Nadine Leconte, et al.. ARE FOODS ENRICHED WITH QUORUM SENSING AUTOINDUCERS CAPABLE OF AFFECTING THE NEONATAL ASSEMBLY OF THE GUT MICROBIOTA IN RATS?. Targeting microbiota : towards clinical revolution, international society of microbiota, Oct 2023, Venise, Italy. ⟨hal-04491014⟩
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