As cycling is becoming more and more integrated in urban mobility policies, cities develop cycling infrastructures to make cycling safer and more enjoyable. Nonetheless cyclists are pretty diverse and have different needs and use of these infrastructures. Using social practice theory (SPT) as the theoretical framework, this paper investigates the meanings associated by cyclists to cycling infrastructures and how they shape their mobility practices. It aims to determine i) what kind of emotions these infrastructures generate among different kind of cyclists, and ii) how these emotions enhance or shift various uses of these infrastructures that are not planned in the making of the infrastructure. I conducted 27 semi-directive interviews with cyclists combined with spatial analysis and on-street observation in the Greater Lyon area. In this communication, I present a typology of cycling infrastructures depending on the meanings and use associated with them. I discuss how the integration of these cycling infrastructures in the urban environment can generate specific emotions and narratives, that in turn generate new uses. By confronting these new uses of the cycling infrastructure to the expectations of policymakers, I show that they are not always anticipated and are often perceived as “misuses”. However, policymakers should consider these meanings and “misuses” in the design of road and dedicated cycling infrastructures.