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Article Dans Une Revue Nature Immunology Année : 2024

Distinct ontogenetic lineages dictate cDC2 heterogeneity

Carlos Minutti
Cécile Piot
  • Fonction : Auteur
Mariana Pereira da Costa
  • Fonction : Auteur
Probir Chakravarty
  • Fonction : Auteur
Neil Rogers
Hector Huerga Encabo
Ana Cardoso
Jane Loong
Gilles Bessou
  • Fonction : Auteur
Jean Langhorne
Dominique Bonnet
Marc Dalod
Elena Tomasello
Caetano Reis E Sousa

Résumé

Abstract Conventional dendritic cells (cDCs) include functionally and phenotypically diverse populations, such as cDC1s and cDC2s. The latter population has been variously subdivided into Notch-dependent cDC2s, KLF4-dependent cDC2s, T-bet + cDC2As and T-bet − cDC2Bs, but it is unclear how all these subtypes are interrelated and to what degree they represent cell states or cell subsets. All cDCs are derived from bone marrow progenitors called pre-cDCs, which circulate through the blood to colonize peripheral tissues. Here, we identified distinct mouse pre-cDC2 subsets biased to give rise to cDC2As or cDC2Bs. We showed that a Siglec-H + pre-cDC2A population in the bone marrow preferentially gave rise to Siglec-H − CD8α + pre-cDC2As in tissues, which differentiated into T-bet + cDC2As. In contrast, a Siglec-H − fraction of pre-cDCs in the bone marrow and periphery mostly generated T-bet − cDC2Bs, a lineage marked by the expression of LysM. Our results showed that cDC2A versus cDC2B fate specification starts in the bone marrow and suggest that cDC2 subsets are ontogenetically determined lineages, rather than cell states imposed by the peripheral tissue environment.

Dates et versions

hal-04479435 , version 1 (27-02-2024)

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Citer

Carlos Minutti, Cécile Piot, Mariana Pereira da Costa, Probir Chakravarty, Neil Rogers, et al.. Distinct ontogenetic lineages dictate cDC2 heterogeneity. Nature Immunology, 2024, ⟨10.1038/s41590-024-01745-9⟩. ⟨hal-04479435⟩

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