Biofuels, which contain oxygenated molecules such as alcohols, work as a promising renewable fuel and tend to limit greenhouse effects and net CO2 emissions. Although these renewable biofuels tend to reduce soot emissions, on the other hand, they cause the formation of other harmful compounds such as aldehydes (formaldehyde, acetaldehyde, etc.). In order to study the effect of biofuels on gasoline, a reference fuel (TRF) representing the gasoline properties composed of iso-octane, n-heptane, and toluene has been studied in a set of experimental measurements to establish the chemical processes of TRF-biofuels on aldehydes, PAHs (polycyclic aromatic hydrocarbons), and soot. Laminar premixed atmospheric flames of TRF fuel mixed with alcoholic biofuels (ethanol and isobutanol) were used in low and high sooting flames stabilized on a Mc Kenna burner at PC2A Laboratory. At the moment, the mole fractions of unsaturated aliphatic species, aldehydes, and other small oxygenated species, mono-aromatics have been investigated using different gas chromatography systems. In the next steps, flame temperature will be measured using a laser diagnostic technique and thermocouple. Soot and PAH measurements will be investigated as well.