Human Serum Albumin in the presence of Small Platinum Nanoparticles
Platinum Nanoparticles Stabilize Human Serum Albumin Structure
Résumé
Noble metals, especially platinum nanoparticles (Pt NPs) have immense potential in nanomedicine as therapeutic agents on account of their high surface area, intrinsic 2 catalytic properties, etc. However, our understanding of the interaction of nanoparticles with biological entities, especially proteins, is far behind the explosive development of nanotechnology. In this study, we measure the structural and stability changes of proteins at nanomolar concentrations upon interaction with Pt NPs using various techniques. A large increase of 18°C in the thermal unfolding of human serum albumin (HSA) with Pt NPs at the molar ratio 1:1 is observed using synchrotron radiation circular dichroism (SRCD), due to Pt NPs increased thermal stability of HSA through preferential hydration processes. The use of SRCD allows measuring critical parameters on NPs-protein interactions, provides additional information for understanding the influential role of PEG coating in NPs-cell interaction and has implications for nanomedicine and nanotoxicology.
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