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Article Dans Une Revue Developmental Cell Année : 2024

Aberrant cortex contractions impact mammalian oocyte quality

Résumé

The cortex controls cell shape. In mouse oocytes, the cortex thickens in an Arp2/3-complex-dependent manner, ensuring chromosome positioning and segregation. Surprisingly, we identify that mouse oocytes lacking the Arp2/3 complex undergo cortical actin remodeling upon division, followed by cortical con- tractions that are unprecedented in mammalian oocytes. Using genetics, imaging, and machine learning, we show that these contractions stir the cytoplasm, resulting in impaired organelle organization and ac- tivity. Oocyte capacity to avoid polyspermy is impacted, leading to a reduced female fertility. We could diminish contractions and rescue cytoplasmic anomalies. Similar contractions were observed in human oocytes collected as byproducts during IVF (in vitro fertilization) procedures. These contractions corre- late with increased cytoplasmic motion, but not with defects in spindle assembly or aneuploidy in mice or humans. Our study highlights a multiscale effect connecting cortical F-actin, contractions, and cyto- plasmic organization and affecting oocyte quality, with implications for female fertility.
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Dates et versions

hal-04473091 , version 1 (22-02-2024)

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Paternité

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Elvira Nikalayevich, Gaëlle Letort, Ghislain de Labbey, Elena Todisco, Anastasia Shihabi, et al.. Aberrant cortex contractions impact mammalian oocyte quality. Developmental Cell, 2024, 59 (7), pp.841-852.e7. ⟨10.1016/j.devcel.2024.01.027⟩. ⟨hal-04473091⟩
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