Modulation of Fecal Clostridiales Bacteria and Butyrate by Probiotic Intervention with Lactobacillus paracasei DG Varies among Healthy Adults - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Nutrition Année : 2014

Modulation of Fecal Clostridiales Bacteria and Butyrate by Probiotic Intervention with Lactobacillus paracasei DG Varies among Healthy Adults

Chiara Ferrario
  • Fonction : Auteur
Valentina Taverniti
  • Fonction : Auteur
Christian Milani
  • Fonction : Auteur
Walter Fiore
  • Fonction : Auteur
Monica Laureati
  • Fonction : Auteur
Ivano de Noni
  • Fonction : Auteur
Milda Stuknyte
  • Fonction : Auteur
Patrizia Riso
  • Fonction : Auteur
Simone Guglielmetti
  • Fonction : Auteur
  • PersonId : 910574

Résumé

Background: The modulation of gut microbiota is considered to be the first target to establish probiotic efficacy in a healthy population. Objective: This study was conducted to determine the impact of a probiotic on the intestinal microbial ecology of healthy volunteers. Methods: High-throughput 16S ribosomal RNA gene sequencing was used to characterize the fecal microbiota in healthy adults (23-55 y old) of both sexes, before and after 4 wk of daily consumption of a capsule containing at least 24 billion viable Lactobacillus paracasei DG cells, according to a randomized, double-blind, crossover placebo-controlled design. Results: Probiotic intake induced an increase in Proteobacteria (P = 0.006) and in the Clostridiales genus Coprococcus (P = 0.009), whereas the Clostridiales genus Blautia (P = 0.036) was decreased; a trend of reduction was also observed for Anaerostipes (P = 0.05) and Clostridium (P = 0.06). We also found that the probiotic effect depended on the initial butyrate concentration. In fact, participants with butyrate >100 mmol/kg of wet feces had a mean butyrate reduction of 49 6 21% and a concomitant decrease in the sum of 6 Clostridiales genera, namely Faecalibacterium, Blautia, Anaerostipes, Pseudobutyrivibrio, Clostridium, and Butyrivibrio (P = 0.021), after the probiotic intervention. In contrast, in participants with initial butyrate concentrations <25 mmol/kg of wet feces, the probiotic contributed to a 329 6 255% (mean 6 SD) increment in butyrate concomitantly with an ;55% decrease in Ruminococcus (P = 0.016) and a 150% increase in an abundantly represented unclassified Bacteroidales genus (P = 0.05). Conclusions: The intake of L. paracasei DG increased the Blautia:Coprococcus ratio, which, according to the literature, can potentially confer a health benefit on the host. The probiotic impact on the microbiota and on short-chain fatty acids, however, seems to strictly depend on the initial characteristics of the intestinal microbial ecosystem. In particular, fecal butyrate concentrations could represent an important biomarker for identifying subjects who may benefit from probiotic treatment. This trial was registered at www.controlled-trials.com/isrctn as ISRCTN56945491.
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Dates et versions

hal-04472171 , version 1 (22-02-2024)

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Chiara Ferrario, Valentina Taverniti, Christian Milani, Walter Fiore, Monica Laureati, et al.. Modulation of Fecal Clostridiales Bacteria and Butyrate by Probiotic Intervention with Lactobacillus paracasei DG Varies among Healthy Adults. Journal of Nutrition, 2014, 144 (11), pp.1787 - 1796. ⟨10.3945/jn.114.197723⟩. ⟨hal-04472171⟩
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