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Article Dans Une Revue Parasites & Vectors Année : 2015

Mutual exclusion of Asaia and Wolbachia in the reproductive organs of mosquito vectors

Paolo Rossi
  • Fonction : Auteur
Irene Ricci
  • Fonction : Auteur
Alessia Cappelli
  • Fonction : Auteur
Claudia Damiani
  • Fonction : Auteur
Ulisse Ulissi
  • Fonction : Auteur
Maria Vittoria Mancini
  • Fonction : Auteur
Matteo Valzano
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Aida Capone
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Sara Epis
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Elena Crotti
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Patrizia Scuppa
  • Fonction : Auteur
Deepak Joshi
  • Fonction : Auteur
Zhiyong Xi
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Mauro Mandrioli
  • Fonction : Auteur
Luciano Sacchi
  • Fonction : Auteur
Scott L O’neill
  • Fonction : Auteur
Guido Favia
  • Fonction : Auteur
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Résumé

Background: Wolbachia is a group of intracellular maternally inherited bacteria infecting a high number of arthropod species. Their presence in different mosquito species has been largely described, but Aedes aegypti, the main vector of Dengue virus, has never been found naturally infected by Wolbachia. Similarly, malaria vectors and other anophelines are normally negative to Wolbachia, with the exception of an African population where these bacteria have recently been detected. Asaia is an acetic acid bacterium stably associated with several mosquito species, found as a dominant microorganism of the mosquito microbiota. Asaia has been described in gut, salivary glands and in reproductive organs of adult mosquitoes in Ae. aegypti and in anophelines. It has recently been shown that Asaia may impede vertical transmission of Wolbachia in Anopheles mosquitoes. Methods: Different methods (PCR and qPCR, monoclonal antibody staining and FISH) have been used to address the question of the co-localization and the relative presence/abundance of the two symbionts. PCR and qPCR were performed to qualitatively and quantitatively verify the distribution of Asaia and Wolbachia in different mosquito species/organs. Monoclonal antibody staining and FISH were performed to localize the symbionts in different mosquito species. Results: Here we provide evidence that, in Anopheles and in other mosquitoes, there is a reciprocal negative interference between Asaia and Wolbachia symbionts, in terms of the colonization of the gonads. In particular, we have shown that in some mosquito species the presence of one of the symbionts prevented the establishment of the second, while in other systems the symbionts were co-localized, although at reduced densities. Conclusions: A mutual exclusion or a competition between Asaia and Wolbachia may contribute to explain the inability of Wolbachia to colonize the female reproductive organs of anophelines, inhibiting its vertical transmission and explaining the absence of Wolbachia infection in Ae. aegypti and in the majority of natural populations of Anopheles mosquitoes.
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Origine : Publication financée par une institution
Licence : CC BY - Paternité

Dates et versions

hal-04470151 , version 1 (21-02-2024)

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Paolo Rossi, Irene Ricci, Alessia Cappelli, Claudia Damiani, Ulisse Ulissi, et al.. Mutual exclusion of Asaia and Wolbachia in the reproductive organs of mosquito vectors. Parasites & Vectors, 2015, 8 (1), pp.278. ⟨10.1186/s13071-015-0888-0⟩. ⟨hal-04470151⟩
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