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Article Dans Une Revue Insect Science Année : 2017

Evidence for a conserved microbiota across the different developmental stages of Plodia interpunctella

Valeria Mereghetti
  • Fonction : Auteur
Lidia Limonta
  • Fonction : Auteur
Daria Patrizia Locatelli
  • Fonction : Auteur
Matteo Montagna

Résumé

Abstract: Diversity and composition of lepidopteran microbiotas are poorly investigated, especially across the different developmental stages. To improve this knowledge, we characterize the microbiota among different developmental stages of the Indian meal moth, Plodia interpunctella, which is considered one of the major pests of commodities worldwide. Using a culture-independent approach based on Illumina 16S rRNA gene sequencing, we characterized the microbiota of four developmental stages: eggs, first-, and last-instar larvae, and adults. A total of 1022 bacterial OTUs were obtained, showing a quite diversified microbiota associated with all the analyzed stages. The microbiotas associated with P. interpunctella resulted almost constant throughout the developmental stages, with approximately 77% of bacterial OTUs belonging to the phylum of Proteobacteria. The dominant bacterial genus is represented by Burkholderia (~64%), followed by Propionibacterium, Delftia, Pseudomonas, and Stenotrophomonas. A core bacterial community, composed of 139 OTUs, was detected in all the developmental stages, among which 112 OTUs were assigned to the genus Burkholderia. A phylogenetic reconstruction, based on the 16S rRNA, revealed that our Burkholderia OTUs clustered with Burkholderia cepacia complex, in the same group as those isolated from the hemipterans Gossyparia spuria and Acanthococcus aceris. The functional profiling, predicted based on the bacterial 16S rRNA, indicates differences in the metabolic pathways related to the metabolism of amino acids between preimaginal and adult stages. We can hypothesize that bacteria may support the insect host during preimaginal stages.
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Licence : CC BY - Paternité

Dates et versions

hal-04470011 , version 1 (21-02-2024)

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Valeria Mereghetti, Bessem Chouaia, Lidia Limonta, Daria Patrizia Locatelli, Matteo Montagna. Evidence for a conserved microbiota across the different developmental stages of Plodia interpunctella. Insect Science, 2017, 26 (3), pp.466-478. ⟨10.1111/1744-7917.12551⟩. ⟨hal-04470011⟩
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