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Article Dans Une Revue eLife Année : 2024

Dynamics of macrophage polarization support Salmonella persistence in a whole living organism

Tamara Sipka
  • Fonction : Auteur
Christina Begon-Pescia
  • Fonction : Auteur
Matteo Bernardello
  • Fonction : Auteur
Sofiane Tairi
  • Fonction : Auteur
Lionello Bossi
  • Fonction : Auteur
Anne-Alicia Gonzalez
  • Fonction : Auteur
Xavier Mialhe
  • Fonction : Auteur
Emilio Gualda
  • Fonction : Auteur
Pablo Loza-Alvarez
Anne Blanc-Potard
  • Fonction : Auteur
Georges Lutfalla
  • Fonction : Auteur
Mai Nguyen-Chi

Résumé

Numerous intracellular bacterial pathogens interfere with macrophage function, including macrophage polarization, to establish a niche and persist. However, the spatiotemporal dynamics of macrophage polarization during infection within host remain to be investigated. Here, we implement a model of persistent Salmonella Typhimurium infection in zebrafish, which allows visualization of polarized macrophages and bacteria in real time at high resolution. While macrophages polarize toward M1-like phenotype to control early infection, during later stages, Salmonella persists inside non-inflammatory clustered macrophages. Transcriptomic profiling of macrophages showed a highly dynamic signature during infection characterized by a switch from pro-inflammatory to anti-inflammatory/pro-regenerative status and revealed a shift in adhesion program. In agreement with this specific adhesion signature, macrophage trajectory tracking identifies motionless macrophages as a permissive niche for persistent Salmonella . Our results demonstrate that zebrafish model provides a unique platform to explore, in a whole organism, the versatile nature of macrophage functional programs during bacterial acute and persistent infections.
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Dates et versions

hal-04467359 , version 1 (20-02-2024)

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Jade Leiba, Tamara Sipka, Christina Begon-Pescia, Matteo Bernardello, Sofiane Tairi, et al.. Dynamics of macrophage polarization support Salmonella persistence in a whole living organism. eLife, 2024, 13, ⟨10.7554/eLife.89828⟩. ⟨hal-04467359⟩
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