Three-dimensional (3D) culture of adult murine colon as an in vitro model of cryptosporidiosis: Proof of concept - Archive ouverte HAL Accéder directement au contenu
Autre Publication Scientifique Scientific Reports Année : 2017

Three-dimensional (3D) culture of adult murine colon as an in vitro model of cryptosporidiosis: Proof of concept

Résumé

Abstract Cryptosporidium parvum is a major cause of diarrheal illness and was recently potentially associated with digestive carcinogenesis. Despite its impact on human health, Cryptosporidium pathogenesis remains poorly known, mainly due to the lack of a long-term culture method for this parasite. Thus, the aim of the present study was to develop a three-dimensional (3D) culture model from adult murine colon allowing biological investigations of the host-parasite interactions in an in vivo- like environment and, in particular, the development of parasite-induced neoplasia. Colonic explants were cultured and preserved ex vivo for 35 days and co-culturing was performed with C . parvum . Strikingly, the resulting system allowed the reproduction of neoplastic lesions in vitro at 27 days post-infection (PI), providing new evidence of the role of the parasite in the induction of carcinogenesis. This promising model could facilitate the study of host-pathogen interactions and the investigation of the process involved in Cryptosporidium -induced cell transformation.

Dates et versions

hal-04466299 , version 1 (19-02-2024)

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Martha Baydoun, Sadia Benamrouz Vanneste, Colette Creusy, Karine Guyot, Nausicaa Gantois, et al.. Three-dimensional (3D) culture of adult murine colon as an in vitro model of cryptosporidiosis: Proof of concept. 2017, pp.17288. ⟨10.1038/s41598-017-17304-2⟩. ⟨hal-04466299⟩
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