Large-scale mapping of the Ebola NP and GP proteins reveals multiple immunoprevalent and conserved CD4 T-cell epitopes - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Cellular and molecular immunology Année : 2020

Large-scale mapping of the Ebola NP and GP proteins reveals multiple immunoprevalent and conserved CD4 T-cell epitopes

Résumé

Humankind faces regular outbreaks from emerging viruses, such as Ebola and the current SARS-CoV-2, or from viruses circulating at low levels in the wild animal reservoir. Owing to the suddenness of virus expansion, the lack of knowledge of immune determinants and correlates of protection severely limit the ability to address the outbreaks. Identified in 1976, Ebola virus is part of the Filoviridae family and causes severe hemorrhagic fever with a mortality rate between 50% and 90%. From 2013 to 2020, more than 30,000 infections and 15,000 deaths have been reported worldwide. High antibody titers against Ebola virus GP are found in patients recovering from Ebola virus infection and correlate with protection in vaccination models.1 Both CD8 and CD4 T-cell responses also seem to participate in the control of Ebola virus infection in animals, and CD4 T cells are necessary to induce resistance to Ebola virus challenge.2 In addition to GP, NP induces strong T-cell responses in animal models that protect against Ebola virus infection, and in convalescent Ebola patients, most of the T-cell responses mounted against Ebola virus were found to target NP.3 However, very few T-cell epitopes specific for both the Ebola GP and NP proteins have yet been identified in humans.4,5 Only one study reported CD4 T-cell epitopes restricted to HLA-DR3.6 We therefore established a large-scale approach to identify CD4 T-cell epitopes using donors not exposed to infection to anticipate the sudden rise of emerging viruses and applied this approach to the Ebola GP and NP proteins.

Domaines

Immunologie
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Dates et versions

hal-04454271 , version 1 (15-03-2024)

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Yann Gallais, Raphaël Sierocki, Gautier Lhomme, Coline Sivelle, Divor Kiseljak, et al.. Large-scale mapping of the Ebola NP and GP proteins reveals multiple immunoprevalent and conserved CD4 T-cell epitopes. Cellular and molecular immunology, 2020, 18 (5), pp.1323-1325. ⟨10.1038/s41423-020-0455-2⟩. ⟨hal-04454271⟩
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