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Communication Dans Un Congrès Année : 2023

Biopeptides derived from red macroalgae protein and their potential as heart health-promoting functional food ingredients

Résumé

Aim: Currently, red seaweed has been demonstrated as an important economic biomass with high contents and quality of protein and essential amino acids, potentially making it an attractive and promising alternative protein source for global food sustainability. Angiotensin-converting enzyme (ACE) has a significant role in the regulation of blood pressure and ACE inhibition with inhibitory peptides is considered as a major target to prevent hypertension. Until now, very few studies on ACE inhibitory peptides from red macroalgae have been conducted. Thus, we investigated the generation, isolation and characterization of ACE inhibitory peptides from Gracilaria gracilis proteins by enzymatic hydrolysis for use as a potential functional food ingredients. Method: Firstly, the red seaweed proteins were extracted using different cell disruption strategies (chemical, physical and enzymatic treatments) applied individually or in combination. The protein content of the raw material and the resulting extracts were determined and amino acid profiling was carried out using an amino acid analyzer. Secondly, G. gracilis proteins were hydrolyzed using various food grade proteases to generate bioactive peptides, and their ACE inhibitory activities were assessed. The G. gracilis protein hydrolysates (GGPHs) were further fractionated and purified by membrane ultrafiltration and reverse-phase high performance liquid chromatography. Fractions and peptides were analysed by mass spectrometry. Furthermore, antihypertensive effects of peptides in spontaneously hypertensive rats (SHRs) were tested. Results: Among the different methods of extraction investigated, it was found that the protein extraction efficiency was significantly increased by the use of cocktail based to proteases and polysaccharide-degrading enzymes compared to when enzyme was used alone. In terms of protein hydrolysis, the alcalase-digested hydrolysates exhibited the highest ACE inhibitory activity. Moreover, the degree of hydrolysis and anti-ACE potential of GGPHs can vary depending on the proteases used. After fractionation and purification by ultrafiltration, the <1 kDa fraction was the most active as inhibitor of the in vitro activities of ACE. Some of the peptides identified revealed strong blood pressure reductions in SHRs. Conclusion: The biopeptides derived from protein hydrolysates of G. gracilis would be a heart health-promoting functional ingredients for nutraceuticals acting against hypertension and its related diseases.
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hal-04451274 , version 1 (11-02-2024)

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  • HAL Id : hal-04451274 , version 1

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Rafik Balti, Jihen Dhaouafi, Naima Nedjar. Biopeptides derived from red macroalgae protein and their potential as heart health-promoting functional food ingredients. 37th EFFoST International Conference 2023 Sustainable Food and Industry 4.0: Towards the 2030 Agenda, European Federation of Food Science and Technology (EFFoST), Nov 2023, Valencia, Spain. ⟨hal-04451274⟩
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