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Article Dans Une Revue Scientific Reports Année : 2014

Modulation properties of factors released by bone marrow stromal cells on activated microglia: an in vitro study

Résumé

Abstract In the present paper we develop a new non-cell based (cell-free) therapeutic approach applied to BV2 microglial cells and spinal cord derived primary microglia (PM) using conditioned media from rat bone marrow stromal cells (BMSCs-CM). First we collected conditioned media (CM) from either naive or injured rat spinal cord tissue (SCI-CM, inflammatory stimulation agent) and from rat bone marrow stromal cells (BMSCs-CM, therapeutic immunomodulation agent). They were both subsequently checked for the presence of chemokines and growth, neurotrophic and neural migration factors using proteomics analysis. The data clearly showed that rat BMSCs-CM contain in vitro growth factors, neural migration factors, osteogenic factors, differentiating factors and immunomodulators, whereas SCI-CM contain chemokines, chemoattractant factors and neurotrophic factors. Afterwards we determined whether the BMSCs-CM affect chemotactic activity, NO production, morphological and pro-apoptotic changes of either BV2 or PM cells once activated with SCI-CM. Our results confirm the anti-migratory and NO-inhibitory effects of BMSCs-CM on SCI-CM-activated microglia with higher impact on primary microglia. The cytotoxic effect of BMSCs-CM occurred only on SCI-CM-stimulated BV2 cells and PM, not on naive BV2 cells, nor on PM. Taken together, the molecular cocktail found in BMSCs-CM is favorable for immunomodulatory properties.

Dates et versions

hal-04449213 , version 1 (09-02-2024)

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Dasa Cizkova, Stéphanie Devaux, Françoise Le Marrec-Croq, Julien Franck, Lucia Slovinska, et al.. Modulation properties of factors released by bone marrow stromal cells on activated microglia: an in vitro study. Scientific Reports, 2014, 4 (1), pp.7514. ⟨10.1038/srep07514⟩. ⟨hal-04449213⟩

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