Venomics survey of six myrmicine ants provides insights into the molecular and structural diversity of their peptide toxins - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Insect Biochemistry and Molecular Biology Année : 2022

Venomics survey of six myrmicine ants provides insights into the molecular and structural diversity of their peptide toxins

Françoise Paquet
Niklas Tysklind
Valérie Troispoux
  • Fonction : Auteur
  • PersonId : 958744
Hadrien Lalägue
  • Fonction : Auteur
  • PersonId : 1349192
Jérôme Orivel

Résumé

Among ants, Myrmicinae represents the most speciose subfamily. The venom composition previously described for these social insects is extremely variable, with alkaloids predominant in some genera while, conversely, proteomics studies have revealed that some myrmicine ant venoms are peptide-rich. Using integrated transcriptomic and proteomic approaches, we characterized the venom peptidomes of six ants belonging to the different tribes of Myrmicinae. We identified a total of 79 myrmicitoxins precursors which can be classified into 38 peptide families according to their mature sequences. Myrmicine ant venom peptidomes showed heterogeneous compositions, with linear and disulfide-bonded monomers as well as dimeric toxins. Several peptide families were exclusive to a single venom whereas some were retrieved in multiple species. A hierarchical clustering analysis of precursor signal sequences led us to divide the myrmicitoxins precursors into eight families, including some that have already been described in other aculeate hymenoptera such as secapin-like peptides and voltage-gated sodium channel (Na V) toxins. Evolutionary and structural analyses of two representatives of these families highlighted variation and conserved patterns that might be crucial to explain myrmicine venom peptide functional adaptations to biological targets. Key contribution This study provides novel insights into both peptide diversity and diversification of myrmicine ant venoms.
Fichier principal
Vignette du fichier
Manuscript-Diversity-Myrmicines.pdf (1.84 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-04446316 , version 1 (15-02-2024)

Licence

Copyright (Tous droits réservés)

Identifiants

Citer

Valentine Barassé, Nathan Téné, Christophe Klopp, Françoise Paquet, Niklas Tysklind, et al.. Venomics survey of six myrmicine ants provides insights into the molecular and structural diversity of their peptide toxins. Insect Biochemistry and Molecular Biology, 2022, 151, pp. 103876. ⟨10.1016/j.ibmb.2022.103876⟩. ⟨hal-04446316⟩
54 Consultations
10 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More