Iontophoresis of treprostinil promotes wound healing in a murine model of scleroderma-related ulcers - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Rheumatology Année : 2022

Iontophoresis of treprostinil promotes wound healing in a murine model of scleroderma-related ulcers

Résumé

Abstract Objective Systemic sclerosis (SSc) is a rare, chronic disease characterized by fibrosis, vascular alterations and digital ulcerations. Few drugs have shown efficacy to enhance wound healing of existing SSc-related ulcers. Local delivery of treprostinil, a prostacyclin analogue, may improve wound healing. The present work aimed first at developing a mouse model of SSc-related ulcerations and second at assessing the effect of iontophoresis of treprostinil on wound healing. Methods We used two murine models of SSc: chemically induced with HOCl, and urokinase-type plasminogen activator receptor (uPAR)-deficient. Excisional wounding was performed on the dorsal midline with a biopsy punch. Animals were randomized into three groups: treated with electrostimulation alone, with treprostinil iontophoresis or untreated. We assessed wound healing over time, as well as skin microvascular reactivity, inflammation, microvessel density and collagen distribution, before wounding and after re-epithelialization. Results uPAR–/– mice, but not HOCl-treated mice, showed impaired wound healing and decreased microvascular reactivity compared with their controls. Treprostinil iontophoresis improved wound healing and microvascular density and decreased inflammation in uPAR–/– mice, while electro-stimulation did not. However, treprostinil had no effect on microvascular reactivity and collagen distribution. Conclusion This study suggests that excisional wounds in uPAR–/– mice are a relevant model of SSc-related ulcers. In addition, treprostinil iontophoresis enhances wound healing in this model. Further work in now needed to show whether this effect translates in humans.
Fichier principal
Vignette du fichier
draft_Proof_hi.pdf (746.66 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Licence : CC BY NC ND - Paternité - Pas d'utilisation commerciale - Pas de modification

Dates et versions

hal-04445911 , version 1 (08-02-2024)

Identifiants

Citer

Sylvain Kotzki, Yann Savina, Raphael Bouvet, Hugo Gil, Sophie Blaise, et al.. Iontophoresis of treprostinil promotes wound healing in a murine model of scleroderma-related ulcers. Rheumatology, 2022, 61 (6), pp.2704-2708. ⟨10.1093/rheumatology/keab852⟩. ⟨hal-04445911⟩

Collections

UGA
0 Consultations
1 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More