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Article Dans Une Revue Journal of Cell Science Année : 2020

AURKA destruction is decoupled from its activity at mitotic exit but essential to suppress interphase activity

Ahmed Abdelbaki
  • Fonction : Auteur
H. Begum Akman
  • Fonction : Auteur
Marion Poteau
  • Fonction : Auteur
Rhys Grant
Giulia Guarguaglini
Catherine Lindon

Résumé

Activity of AURKA is controlled through multiple mechanisms including phosphorylation, ubiquitin-mediated degradation, and allosteric interaction with TPX2. Activity peaks at mitosis before AURKA is degraded during and after mitotic exit in a process strictly dependent on APC/C coactivator FZR1. We used FZR1 knockout cells (FZR1KO) and a novel FRET-based AURKA biosensor to investigate how activity is regulated in absence of destruction. We found that AURKA activity in FZR1KO cells dropped at mitotic exit as rapidly as in parental cells, despite absence of destruction. Unexpectedly, TPX2 was degraded normally in FZR1KO cells. Overexpression of an N-terminal TPX2 fragment sufficient for AURKA binding, but not degraded at mitotic exit, caused delay in AURKA inactivation. We conclude that AURKA inactivation at mitotic exit is determined not by its own degradation but by degradation of TPX2 and therefore dependent on CDC20 rather than FZR1. The biosensor revealed that FZR1 instead suppresses AURKA activity in interphase and is critically required for assembly of the interphase mitochondrial network after mitosis.
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Origine : Publication financée par une institution

Dates et versions

hal-04444517 , version 1 (07-02-2024)

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Ahmed Abdelbaki, H. Begum Akman, Marion Poteau, Rhys Grant, Olivier Gavet, et al.. AURKA destruction is decoupled from its activity at mitotic exit but essential to suppress interphase activity. Journal of Cell Science, 2020, ⟨10.1242/jcs.243071⟩. ⟨hal-04444517⟩
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