De novo sequencing and assembly of complex genomes
Résumé
The introduction of short-reads technologies ten years ago, significantly increased the number of available genomes. Generally, these assemblies are incomplete and fragmented, and only a few of them are at the chromosome-scale. More recently, Oxford Nanopore sequencing technology was commercialized with the promise to sequence long DNA fragments (kilobases to megabases order) and then, by using efficient algorithms, provide assembly of high quality in terms of contiguity and completeness of the repetitive regions. This presentation will provide an overview of the capability of current technologies to generate chromosome-scale assemblies and describe some recent projects of plant and animal genomes.