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Article Dans Une Revue Nature Communications Année : 2024

Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall

Résumé

Microalgae are a renewable and promising biomass for large-scale biofuel, food and nutrient production. However, their efficient exploitation depends on our knowledge of the cell wall composition and organization as it can limit access to high-value molecules. Here we provide an atomic-level model of the non-crystalline and water-insoluble glycoprotein-rich cell wall of Chlamydomonas reinhardtii . Using in situ solid-state and sensitivity-enhanced nuclear magnetic resonance, we reveal unprecedented details on the protein and carbohydrate composition and their nanoscale heterogeneity, as well as the presence of spatially segregated protein- and glycan-rich regions with different dynamics and hydration levels. We show that mannose-rich lower-molecular-weight proteins likely contribute to the cell wall cohesion by binding to high-molecular weight protein components, and that water provides plasticity to the cell-wall architecture. The structural insight exemplifies strategies used by nature to form cell walls devoid of cellulose or other glycan polymers.
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hal-04434789 , version 1 (02-02-2024)

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Alexandre Poulhazan, Alexandre Arnold, Frederic Mentink-Vigier, Artur Muszyński, Parastoo Azadi, et al.. Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall. Nature Communications, 2024, 15 (1), pp.986. ⟨10.1038/s41467-024-45246-7⟩. ⟨hal-04434789⟩
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