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Communication Dans Un Congrès Année : 2022

A new family of structurally conserved fungal effectors displays epistatic interactions with plant resistance proteins

Résumé

Leptosphaeria maculans, causal agent of oilseed rape stem canker, is one of the few fungal pathogens where suppression of resistance-mediated recognition by an avirulence (AVR) effector has been demonstrated. Indeed, AvrLm4-7 suppresses Rlm3-and Rlm9-mediated resistance triggered by AvrLm3 and AvrLm5-9, respectively. To decipher the epistatic interaction between these L. maculans AVR effectors, we determined the crystal structure of AvrLm5-9 and obtained a 3D model of AvrLm3, based on the crystal structure of Ecp11-1, an AVR effector from Fulvia fulva. Despite a lack of sequence similarity, AvrLm5-9 and AvrLm3 are structural analogues of AvrLm4-7. Structure-informed sequence database searches identified a larger number of putative structural analogues among L. maculans effectors, including the avirulence effector AvrLmS-Lep2, as well as among effectors from other phytopathogenic fungi. Remarkably, transformants of L. maculans expressing one of these structural analogues, Ecp11-1, triggered oilseed rape immunity in several cultivars carrying Rlm3. Furthermore, this resistance could be suppressed by AvrLm4-7.
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Dates et versions

hal-04432974 , version 1 (13-02-2024)

Identifiants

  • HAL Id : hal-04432974 , version 1

Citer

Isabelle Fudal. A new family of structurally conserved fungal effectors displays epistatic interactions with plant resistance proteins. CAnFunNet Fungal Biology Conference, May 2022, Virtual Event (Canada), France. ⟨hal-04432974⟩
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