Optical Trapping and Fast Discrimination of Label‐Free Bacteriophages at the Single Virion Level - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Small Année : 2024

Optical Trapping and Fast Discrimination of Label‐Free Bacteriophages at the Single Virion Level

Résumé

Abstract There is a recent resurgence of interest in phage therapy (the therapeutic use of bacterial viruses) as an approach to eliminating difficult‐to‐treat infections. However, existing approaches for therapeutic phage selection and virulence testing are time‐consuming, host‐dependent, and facing reproducibility issues. Here, this study presents an innovative approach wherein integrated resonant photonic crystal (PhC) cavities in silicon are used as optical nanotweezers for probing and manipulating single bacteria and single virions with low optical power. This study demonstrates that these nanocavities differentiate between a bacterium and a phage without labeling or specific surface bioreceptors. Furthermore, by tailoring the spatial extent of the resonant optical mode in the low‐index medium, phage distinction across phenotypically distinct phage families is demonstrated. The work paves the road to the implementation of optical nanotweezers in phage therapy protocols.

Dates et versions

hal-04431362 , version 1 (01-02-2024)

Identifiants

Citer

Nicolas Villa, Enrico Tartari, Simon Glicenstein, Hugues de Villiers de la Noue, Emmanuel Picard, et al.. Optical Trapping and Fast Discrimination of Label‐Free Bacteriophages at the Single Virion Level. Small, 2024, ⟨10.1002/smll.202308814⟩. ⟨hal-04431362⟩
5 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More