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Article Dans Une Revue Bioinformatics Année : 2021

Cheetah-MS: a web server to model protein complexes using tandem cross-linking mass spectrometry data

Résumé

Abstract Summary Protein–protein interactions (PPIs) are central in many biological processes but difficult to characterize, especially in complex, unfractionated samples. Chemical cross-linking combined with mass spectrometry (MS) and computational modeling is gaining recognition as a viable tool in protein interaction studies. Here, we introduce Cheetah-MS, a web server for predicting the PPIs in a complex mixture of samples. It combines the capability and sensitivity of MS to analyze complex samples with the power and resolution of protein–protein docking. It produces the quaternary structure of the PPI of interest by analyzing tandem MS/MS data (also called MS2). Combining MS analysis and modeling increases the sensitivity and, importantly, facilitates the interpretation of the results. Availability and implementation Cheetah-MS is freely available as a web server at https://www.txms.org.
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Dates et versions

hal-04430468 , version 1 (31-01-2024)

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Hamed Khakzad, Lotta Happonen, Johan Malmström, Lars Malmström. Cheetah-MS: a web server to model protein complexes using tandem cross-linking mass spectrometry data. Bioinformatics, 2021, 37 (24), pp.4871-4872. ⟨10.1093/bioinformatics/btab449⟩. ⟨hal-04430468⟩
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