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Autre Publication Scientifique Scientific Reports Année : 2017

Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge

Résumé

Abstract Precise localization of nanoparticles within a cell is crucial to the understanding of cell-particle interactions and has broad applications in nanomedicine. Here, we report a proof-of-principle experiment for imaging individual functionalized nanoparticles within a mammalian cell by correlative microscopy. Using a chemically-fixed HeLa cell labeled with fluorescent core-shell nanoparticles as a model system, we implemented a graphene-oxide layer as a substrate to significantly reduce background scattering. We identified cellular features of interest by fluorescence microscopy, followed by scanning transmission X-ray tomography to localize the particles in 3D, and ptychographic coherent diffractive imaging of the fine features in the region at high resolution. By tuning the X-ray energy to the Fe L-edge, we demonstrated sensitive detection of nanoparticles composed of a 22 nm magnetic Fe 3 O 4 core encased by a 25-nm-thick fluorescent silica (SiO 2 ) shell. These fluorescent core-shell nanoparticles act as landmarks and offer clarity in a cellular context. Our correlative microscopy results confirmed a subset of particles to be fully internalized, and high-contrast ptychographic images showed two oxidation states of individual nanoparticles with a resolution of ~16.5 nm. The ability to precisely localize individual fluorescent nanoparticles within mammalian cells will expand our understanding of the structure/function relationships for functionalized nanoparticles.

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Chimie
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Dates et versions

hal-04424355 , version 1 (15-04-2024)

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Marcus Gallagher-Jones, Carlos Sato Baraldi Dias, Alan Pryor, Karim Bouchmella, Lingrong Zhao, et al.. Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge. 2017, pp.4757. ⟨10.1038/s41598-017-04784-5⟩. ⟨hal-04424355⟩
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