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Article Dans Une Revue Journal of General Virology Année : 2014

Genome diversity and evidence of recombination and reassortment in nanoviruses from Europe

Ioana Grigoras
Ana Isabel del Cueto Ginzo
  • Fonction : Auteur
Darren Martin
  • Fonction : Auteur
Arvind Varsani
  • Fonction : Auteur
Javier Romero
  • Fonction : Auteur
Alamdar Ch. Mammadov
  • Fonction : Auteur
Irada Huseynova
  • Fonction : Auteur
Jalal Aliyev
  • Fonction : Auteur
Ahmed Kheyr-Pour
  • Fonction : Auteur
Herbert Huss
  • Fonction : Auteur
Heiko Ziebell
  • Fonction : Auteur
Heinrich-Josef Vetten
  • Fonction : Auteur

Résumé

The recent identification of a new nanovirus, pea necrotic yellow dwarf virus, from pea in Germany prompted us to survey wild and cultivated legumes for nanovirus infections in several European countries. This led to the identification of two new nanoviruses: black medic leaf roll virus (BMLRV) and pea yellow stunt virus (PYSV), each considered a putative new species. The complete genomes of a PYSV isolate from Austria and three BMLRV isolates from Austria, Azerbaijan and Sweden were sequenced. In addition, the genomes of five isolates of faba bean necrotic yellows virus (FBNYV) from Azerbaijan and Spain and those of four faba bean necrotic stunt virus (FBNSV) isolates from Azerbaijan were completely sequenced, leading to the first identification of FBNSV occurring in Europe. Sequence analyses uncovered evolutionary relationships, extensive reassortment and potential remnants of mixed nanovirus infections, as well as intra- and intercomponent recombination events within the nanovirus genomes. In some virus isolates, diverse types of the same genome component (paralogues) were observed, a type of genome complexity not described previously for any member of the family Nanoviridae . Moreover, infectious and aphid-transmissible nanoviruses from cloned genomic DNAs of FBNYV and BMLRV were reconstituted that, for the first time, allow experimental reassortments for studying the genome functions and evolution of these nanoviruses.

Dates et versions

hal-04415793 , version 1 (24-01-2024)

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Citer

Ioana Grigoras, Ana Isabel del Cueto Ginzo, Darren Martin, Arvind Varsani, Javier Romero, et al.. Genome diversity and evidence of recombination and reassortment in nanoviruses from Europe. Journal of General Virology, 2014, 95 (5), pp.1178-1191. ⟨10.1099/vir.0.063115-0⟩. ⟨hal-04415793⟩
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