Road‐traffic‐related air pollution contributes to skin barrier alteration and growth defect of sensory neurons - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Experimental Dermatology Année : 2024

Road‐traffic‐related air pollution contributes to skin barrier alteration and growth defect of sensory neurons

Résumé

Abstract The effects of air pollution on health are gaining increasing research interest with limited data on skin alterations available. It was suggested that air pollution is a trigger factor for sensitive skin (SS). However, this data was based on surveys with a lack of experimental data. SS is related to altered skin nerve endings and cutaneous neurogenic inflammation. TTe present study was to assess the in vitro effect of particulate matter (PM) on epidermis and nerve ending homeostasis. PM samples were collected according to a validated protocol. Reconstructed human epidermis (RHE, Episkin®) was exposed to PM and subsequently the supernatants were transferred to a culture of PC12 cells differentiated into sensory neurons (SN). Cell viability, axonal growth and neuropeptide‐release were measured. The modulation of the expression of different inflammatory, keratinocytes differentiation and neurites growth markers was assessed. PM samples contained a high proportion of particles with a size below 1 μm and a complex chemical composition. Transcriptomic and immunohistochemical analyses revealed that PM altered keratinocytes terminal differentiation and induced an inflammatory response. While viability and functionality of the SN were not modified, their outgrowth was significantly decreased after incubation with PM‐exposed Episkin® supernatants. This was closely related to the modification of nerve growth factor/semaphorin 3A balance. This study showed that air pollutants have negative effects on keratinocytes and sensory nerve endings including inflammatory responses. These effects are probably involved in the SS pathophysiology and might be involved in inflammatory skin disorders.
Fichier non déposé

Dates et versions

hal-04411208 , version 1 (23-01-2024)

Identifiants

Citer

Christelle Le Gall-Lanotto, Anthony Verdin, Fabrice Cazier, Adeline Bataille-Savattier, Christelle Guéré, et al.. Road‐traffic‐related air pollution contributes to skin barrier alteration and growth defect of sensory neurons. Experimental Dermatology, 2024, 33 (1), pp.e15009. ⟨10.1111/exd.15009⟩. ⟨hal-04411208⟩
27 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More