Development of an in vitro coculture of primary sensitive pig neurons and keratinocytes for the study of cutaneous neurogenic inflammation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Experimental Dermatology Année : 2010

Development of an in vitro coculture of primary sensitive pig neurons and keratinocytes for the study of cutaneous neurogenic inflammation

Ulysse Pereira
Nicholas Boulais
  • Fonction : Auteur
Nicholas Lebonvallet
  • Fonction : Auteur
Luc Lefeuvre
  • Fonction : Auteur
Agnès Gougerot
  • Fonction : Auteur
Laurent Misery
  • Fonction : Auteur

Résumé

Please cite this paper as: Development of an in vitro coculture of primary sensitive pig neurons and keratinocytes for the study of cutaneous neurogenic inflammation. Experimental Dermatology 2010; 19 : 931–935. Abstract: Cutaneous neurogenic inflammation (CNI) is often associated with skin disorders. Activated sensory neurons secrete neuropeptides, such as substance P (SP), which initiate or aggravate inflammation in the skin. The discovery of new molecules acting on these neurons is hampered by the difficulty of reproducing the interactions between nerve endings and skin in vitro . We developed an in vitro model based on the coculture of porcine primary keratinocytes and sensory neurons, which mimics skin innervation. To test the relevance of this model, we compared the effects of different substances on CNI by measuring SP secretion in vitro using a sensitive enzyme immunoassay. Collectively, our results indicate that the use of porcine cells could be very useful to perform an in vitro model of CNI. By adding capsaicin, which induces the secretion of SP by neurons, to the culture, we show that our model mimics CNI in vitro , allowing us to screen for molecules that inhibit this inflammatory response. Such a model can be used to test the effects of different substances on CNI and may be useful for dermatological or cosmetic applications. Based on our screen, we found that extracts of Laminaria digitata and Vernonia sublutea inhibit CNI.

Domaines

Biotechnologies

Dates et versions

hal-04404165 , version 1 (18-01-2024)

Identifiants

Citer

Ulysse Pereira, Nicholas Boulais, Nicholas Lebonvallet, Luc Lefeuvre, Agnès Gougerot, et al.. Development of an in vitro coculture of primary sensitive pig neurons and keratinocytes for the study of cutaneous neurogenic inflammation. Experimental Dermatology, 2010, 19 (10), pp.931-935. ⟨10.1111/j.1600-0625.2010.01119.x⟩. ⟨hal-04404165⟩
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